RESUMEN
The resilience of transport systems, facing natural or man-made disruptions, has been widely discussed in literature in terms of recovery capabilities concerning infrastructures, suggesting solutions to provide users an acceptable level of service along the interrupted network. However, in the context of the Covid-19 outbreak, the disruption has stressed the resilience of transport systems not on the supply side but rather at organizational level for transport service providers. Indeed, the sudden and drastic decrease in users due to the restrictions imposed by governments to limit the pandemic spread has implicated severe economic consequences in the running of transport companies. In this paper, attention has been focused on the public transport sector to analyse the effects of different initiatives, which companies could undertake in response to the demand shock caused by the Covid-19 emergency. Notably, an optimization procedure has been developed with the aim of determining feasible Pareto-front solutions, which correspond to trade-off conditions for the concurrent maximization of the company profit and the minimization of outsourcing services. The time span necessary to implement the examined recovery measures has been considered together with the limitation to appropriate threshold values for the main cost and income items influencing the company operations management. The proposed approach has been applied to the case study of an Italian public transport company to appraise different post-Covid-19 resilience strategies.
RESUMEN
BACKGROUND: Standardized methods for testing Viral Filtration Efficiency (VFE) of tissues and devices are lacking and few studies are available on aerosolizing, sampling and assessing infectivity of SARS-CoV-2 in controlled laboratory settings. NanoAg-coated endonasal filters appear a promising aid for lowering viable virus inhalation in both adult and younger populations (e.g., adolescents). OBJECTIVE: to provide an adequate method for testing SARS-CoV-2 bioaerosol VFE of bio-gel Ag nanoparticles endonasal filters, by a model system, assessing residual infectivity as cytopathic effect and viral proliferation on in vitro cell cultures. METHODS: A SARS-CoV-2 aerosol transmission chamber fed by a BLAM aerosol generator produces challenges (from very high viral loads (105 PFU/mL) to lower ones) for endonasal filters positioned in a Y shape sampling port connected to a Biosampler. An aerosol generator, chamber and sampler are contained in a class II cabinet in a BSL3 facility. Residual infectivity is assessed from aliquots of liquid collecting bioaerosol, sampled without and with endonasal filters. Cytopathic effect as plaque formation and viral proliferation assessed by qRT-PCR on Vero E6 cells are determined up to 7 days post inoculum. RESULTS: Each experimental setting is replicated three times and basic statistics are calculated. Efficiency of aerosolization is determined as difference between viral load in the nebulizer and in the Biosampler at the first day of experiment. Efficiency of virus filtration is calculated as RNA viral load ratio in collected bioaerosol with and without endonasal filters at the day of the experiment. Presence of infectious virus is assessed by plaque forming unit assay and RNA viral load variations. CONCLUSIONS: A procedure and apparatus for assessing SARS-CoV-2 VFE for endonasal filters is proposed. The apparatus can be implemented for more sophisticated studies on contaminated aerosols.